Image: The Troponin T hs assay (Photo courtesy of Roche Diagnostics).
Galectin-3 is a unique member of chimera type galectins and is involved in a large number of disease processes and it participates in cell adhesion, activation, proliferation, apoptosis as well as cell migration. To date, only few and conflicting data have been published on upper reference limits (URL) of circulating galectin-3.
The determination of upper reference limits (URL) for galectin-3, high-sensitivity cardiac troponin I (hs-cTnI) and high-sensitivity cardiac troponin T (hs-cTnT) in a single cohort of healthy blood donors using routine assays has been described. Reference ranges for blood tests are sets of values used by a health professional to interpret a set of medical test results from blood samples.
Laboratory medical scientists at the Konventhospital Barmherzige Brueder Linz (Austria) used for the reference values a cohort of 402 consecutive blood donors (64% were male and 36% were female). The median individuals'' age was 35.0 years (range, 18.0 to 64.4). Individuals of this reference population were free of cardiovascular disease, diabetes mellitus, renal disease, cancer, current infection and chronic inflammatory disease.
Plasma concentrations of galectin-3 were measured with the “routine Galectin-3” assay (Abbott Diagnostics, Abbott Park, IL, USA), of hs-cTnI with Abbott Diagnostics’ “STAT High Sensitive Troponin-I” assay, and of hs-cTnT with the “Troponin T hs” assay (Roche Diagnostics, Mannheim, Germany). An Abbott ARCHITECT I2000SR Immunoassay Analyzer was used were appropriate. URLs were calculated by using a non-parametric percentile method.
The 97.5th percentile URL for galectin-3 was 16 ng/mL in males and 17 ng/mL in females; the 99th percentile URL for hs-cTnI was 39 ng/L in males and 24 ng/L in females; and the 99th percentile URL for hs-cTnT was 14 ng/L in males and 11 ng/L in females. The eight individuals with hs-cTnI values equal to or greater than 15 ng/L were different from the seven individuals with hs-cTnT values equal to or greater than 10 ng/L. Of the 402 individuals, none had galectin-3 values below the limit of detection (LOD, less than 1.0 ng/mL, 290 (72%) had hs-cTnI values below the LOD which was 1.9 ng/L, and 359 (89%) had hs-cTnT values below the LOD of 5.0 ng/L.
The authors concluded that plasma concentrations of galectin-3, hs-cTnI and hs-cTnT and corresponding 99th percentile URLs were rather low in their cohort of healthy blood donors compared with previously published data. In their reference population, analyte plasma concentrations above the LOD were detectable in 100% of the individuals with the Abbott galectin-3 assay, but only in less than 50% for both the Abbott hs-cTnI assay and the Roche hs-cTnT assay. The study was originally published online on February 24, 2016, in the journal Clinica Chimica Acta.Related Links:
Konventhospital Barmherzige Brueder LinzAbbott DiagnosticsRoche Diagnostics